ELISA Guinea Tissue inhibitors of metalloproteinase 1 (TIMP1)

Reactivity:Guinea pig (Cavia) UniProt:N/A Abbreviation:TIMP-1 Alternative Names:RP1-230G1.3; CLGI; EPA; EPO; FLJ90373; HCI; TIMP; erythroid potentiating activity;fibroblast collagenase inhibitor;Metalloproteinase inhibitor 1; TIMP1 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.122 ng/mL Intra-AssayCV:?4.3% Inter-AssayCV:?7.5% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TIMP-1 in samples. An antibody specific for TIMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TIMP-1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TIMP metallopeptidase inhibitor 1, a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellµLar matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes sµggests that this gene inactivation is polymorphic in females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).