ELISA Glia-derived nexin (SERPINE2)

Reactivity: (Homo sapiens) UniProt:P07093 Abbreviation:SERPINE2 Alternative Names:GDN; PI7; PN1; PNI; OTTHUMP00000203766|glial-derived neurite promoting factor|glial-derived nexin 1|plasminogen activator inhibitor type 1; member 2|protease inhibitor 7 (protease nexin I)|serine (o Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.062 ng/mL Intra-AssayCV:?6.3% Inter-AssayCV:?9.9% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SERPINE2 in samples. An antibody specific for SERPINE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SERPINE2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINE2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Protease nexin I is a 44-kD thrombin and urokinase inhibitor released by foreskin fibroblasts. PN-I shares several features with antithrombin III , an abundant plasma thrombin inhibitor. Both PN-I and AT-III have high affinities for heparin, and heparin accelerates their rate of thrombin inhibition. In addition, the published sequence of 28 amino acids at the N-terminus of PN-I is identical to the N-terminal amino acid sequence of a glial-derived neurite promoting factor. Two forms, designated alpha and beta, were identified. These differed only by the insertion of 3 nucleotides into the coding sequence, and apparently arose by utilization of an alternative splice acceptor site in the PN1 gene. In the resµLting proteins, alpha-PN-I contains an arginine residue at position 310, whereas beta-PN-I contains threonine-lysine residues. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).