ELISA Glutamine and serine-rich protein 1 (QSER1)

Reactivity: (Homo sapiens) UniProt:Q2KHR3 Abbreviation:QSER1 Alternative Names:FLJ21924; Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.2% Inter-AssayCV:?9.7% Recovery:0.92 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate QSER1 in samples. An antibody specific for QSER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQSER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for QSER1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QSER1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:QSER1 protein contains two high conserved domains found not only in QSER1 but also in other protein products. This property was conserved from the QSER1 to the Coelacanth QSER1. MµLtiple conserved nuclear localization signals were also predicted within the QSER1 protein by pSORT. Predictions of the QSER1 protein structure indicate that the protein contains many alpha helices. NCBI cBLAST predicted structural similarity between the QSER1 protein and the Schizosaccharomyces pombe (fission yeast) RNA Polymerase II A chain. The two regions of similarity occur between amino acids 56-194 and 322-546. This first region (56-194) is a regµLatory region in both the and yeast RNA Polymerase II containing mµLtiple repeats of the sequence YSPTSPSYS. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).