ELISA Glycogen phosphorylase, muscle form (PYGM)

Reactivity: (Homo sapiens) UniProt:P11217 Abbreviation:PYGM Alternative Names:muscle glycogen phosphorylase|myophosphorylase Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.115 ng/mL Intra-AssayCV:?5.6% Inter-AssayCV:?9.5% Recovery:1.09 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PYGM in samples. An antibody specific for PYGM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYGM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PYGM is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYGM bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Mutations in the muscle isoform of glycogen phosphorylase (PYGM) are associated with McArdle disease (glycogen storage disease type V). More than 65 mutations in the PYGM gene that lead to McArdle disease have been identified to date.Gautron et al. (1987) isolated muscle phosphorylase cDNA clones from a cDNA library. Northern blot experiments revealed 1 specific mRNA of 3.4 kb found uniquely in tissues expressing muscle phosphorylase. The muscle glycogen phosphorylase protein comprises 842 amino acids (Kubisch et al., 1998).Burke et al. (1987) determined the intron/exon structure of the PYGM gene. Kubisch et al. (1998) provided a revised genomic structure for the PYGM gene, which contains 20 exons. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).