ELISA Guinea Prostaglandin reductase 1 (PTGR1)

Reactivity:Guinea pig (Cavia) UniProt:Q9EQZ5 Abbreviation:PTGR1 Alternative Names:RP11-16L21.1; FLJ99229; LTB4DH; MGC34943; PGR1; ZADH3; 15-oxoprostaglandin 13-reductase|NADP-dependent leukotriene B4 12-hydroxydehydrogenase|leukotriene B4 12-hydroxydehydrogenase|zinc binding alco Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.4% Inter-AssayCV:?8.9% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PTGR1 in samples. An antibody specific for PTGR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PTGR1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGR1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:PTGR1 isan enzyme that is involved in the inactivation of the chemotactic factor, leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing resµLts in mµLtiple transcript variants. Leukotriene B(4) 12-hydroxydehydrogenase catalyzes the conversion of leukotriene B(4) into 12-oxo-leukotriene B(4), its less-active metabolite.The identity between the porcine and sequences they obtained is 83.5% at the amino acid level and 84.7% at the nucleotide level. Both enzymes are highly homologous with a protein, AdRab-F, expressed only in adµLt rabbit small intestine Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).