ELISA Guinea Troponin C, slow skeletal and cardiac muscles (TNNC2)

Reactivity:Guinea pig (Cavia) UniProt:N/A Abbreviation:TNNC1 Alternative Names:CMD1Z; TNC; TNNC; cardiac troponin C|slow twitch skeletal/cardiac muscle troponin C|troponin C; slow|troponin C1; slow Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?7.2% Inter-AssayCV:?9.6% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TNNC1 in samples. An antibody specific for TNNC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TNNC1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNC1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The troponin C protein is the calcium-binding subunit of the troponin complex. It occurs in 2 distinct isoforms: fast skeletal troponin C, which is expressed exclusively in fast-twitch skeletal muscle, and slow/cardiac troponin C, which is expressed in both cardiac and slow-twitch skeletal muscle. Gahlmann and Kedes (1990) described the structure and sequence of the gene for fast-twitch troponin C.By PCR-based analysis of a monochromosomal hybrid panel followed by analysis of the Genebridge 4 radiation hybrid panel, Townsend et al. (1997) mapped the TNNC2 gene to chromosome 20. Tiso et al. (1997) independently mapped the TNNC2 gene to 20q12-q13.11 using PCR of radiation hybrids. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).