Mycoplasma TIES
The Mycoplasma TIES kit is a highly effective diagnostic tool designed for the identification and differentiation of Ureaplasma spp.. This kit includes a selective medium and 20 test strips for accurate, efficient mycoplasma detection. It uses a combination of urease and arginase activity to differentiate species based on their ability to decompose urea and arginine, releasing ammonia (NH3) that increases the pH and results in a color change of the indicator.
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Internal Reference:
MYCO-GEN-01
Website URL:
/shop/myco-gen-01-mycoplasma-ties-620079
- Selective Medium : The kit contains a mixed medium prepared by combining lyophilized powder with the diluent, ideal for cultivating Ureaplasma spp..
- Precise Identification : The color change of the culture broth is used to detect the presence of urease in Ureaplasma parvum (UP) and Ureaplasma urealyticum (UU), distinguishing them based on their differing tolerance to manganese ions.
- Comprehensive Test Strips : The test strips include 12 antimicrobial agents, allowing for the detection of mycoplasma susceptibility to these agents.
- Color Indicator : The pH increase due to NH3 release changes the color of the broth, providing clear, visible results.
- Enzyme Inhibition : If the mycoplasma is susceptible to the antimicrobial agents, enzyme activity is inhibited, preventing color change.
How It Works :
- Prepare the mixed medium by combining the lyophilized powder with the diluent.
- Cultivate the sample with the medium.
- Monitor the pH change due to the NH3 release, which indicates urease and arginase activity.
- Distinguish Ureaplasma spp. based on color changes and their tolerance to manganese ions.
- The activity of antimicrobial agents in the strip helps determine mycoplasma susceptibility, inhibiting enzyme activity if the strain is susceptible.
Applications
- Mycoplasma Identification: Ideal for identifying Ureaplasma parvum (UP) and Ureaplasma urealyticum (UU), two species of Ureaplasma.
- Antimicrobial Resistance Studies: The antimicrobial agents on the strip help in determining the susceptibility of mycoplasma to a wide range of antibiotics.
- Diagnostics: An essential tool for research in microbiology and clinical diagnostics.